Center for Biomedical & Life Sciences

Cell and Tissue Culture

  • Maintenance of various cell lines
  • Establishment of primary neuronal cultures (e.g.
  • hippocampal, cortical, trigeminal ganglia, spinal cord, etc.)
  • Immunocytochemistry/Immunohistochemistry with fluorescence microscopy
  • Cryostat sectioning of tissue
  • Live Ca/Na cell imaging
  • In vivo experiments and imaging

Molecular Techniques

Polymerase chain reaction (PCR), qPCR, rtPCR
Microarray analysis (protein, DNA)
Gene expression analysis
DNA plasmid transfections and promoter studies
Protein and DNA gel electrophoresis (SDS-PAGE)
Radioimmunossay (RIA)
Enzyme-Linked ImmunoSorbent Assay (ELISA)

Microbiological Techniques

Antimicrobial Assays
Microbial Identification and Quantification
Plasmid Isolation and Purification
Molecular Cloning and Transformation
Restriction Fragment Length Polymorphism (RFLP) Analysis
DNA Sequencing
Microbial Community Analysis
Denaturing Gradient Gel Electrophoresis (DGGE)
Biofilm Formation and Analysis
Probiotic/Dietary Supplement Research and Development
Product/Comparative Testing
Light and Electron Microscopy

Chemical Techniques

Liquid chromatography/Mass spectrometry (LCMS)
Gas chromatography/Mass Mass spectrometry (GCMS)
Ultra high performance liquid chromatography (HPLC)
Isolation and characterization of biological compounds