Center for Biomedical & Life Sciences

Cell and Molecular Techniques

Maintenance of cancer cell lines
Establishment of primary neuronal cultures (e.g. hippocampal, cortical, trigeminal ganglia, spinal cord, etc.)
Immunocytochemistry/Immunohistochemistry with fluorescence microscopy
Analysis of changes in intracellular calcium and sodium ions
Polymerase chain reaction (PCR), qPCR, rtPCR
Microarray analysis (protein, DNA)
Gene expression analysis
DNA plasmid transfections and promoter studies
Protein and DNA gel electrophoresis (SDS-PAGE)
Radioimmunoassay (RIA)
Enzyme-Linked ImmunoSorbent Assay (ELISA)

Preclinical Models of Migraine and TMD

Acute and chronic models
Inflammatory and non-inflammatory models
Behavioral studies for anxiety and stress
Behavioral testing for mechanical, thermal, and chemical sensitivity/activation
Auditory testing including tone burst ABR and study of neural pathways

Microbiological Techniques

Antimicrobial Assays
Microbial Identification and Quantification
Plasmid Isolation and Purification
Molecular Cloning and Transformation
Microbial Community Analysis
Biofilm Formation and Analysis
Probiotic/Dietary Supplement Research and Development
Product/Comparative Testing
Gram staining, light and electron microscopy

Chemical Techniques

Extraction and fractionation
Electrolyte analysis in biological fluids
Colorimetric assays